fasterp WASM Sandbox

Controls

Loading WASM...

Length Filtering

Min Length

Max Length (0 = no limit)

Quality Filtering

N Base Limit

Quality Threshold (Phred)

Unqualified % Limit

Sliding Window

Mean Quality

Window Size

Cut Front

Cut Tail

Poly Trimming

Trim Poly-G

Trim Poly-X

Input FASTQ

@READ_1 good quality read
GATTTGGGGTTCAAAGCAGTATCGATCAAATAGTAAATCCATTTGTTCAACTCACAGTTT
+
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@READ_2 all N bases - will be filtered
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@READ_3 low quality - will be filtered
ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTACGT
+
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
@READ_4 good quality read
GCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTAGCTA
+
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
@READ_5 too short - will be filtered
ATCGATCGATCG
+
IIIIIIIIIIII
@READ_6 poly-G tail - will be trimmed
ACGTACGTACGTACGTACGTACGTACGTACGTACGTACGTGGGGGGGGGGGGGGGGGGGG
+
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@READ_7 good quality read
TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
+
JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ